Open AccessCoordinate induction of peroxidase and H 2 O 2 ‐forming nucleoside oxidase in Flavobacterium meningosepticum
Author(s) -
Koga Shinji,
Ogawa Jun,
Choi YangMun,
Shimizu Sakayu
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13609.x
Subject(s) - peroxidase , nucleoside , biochemistry , oxidase test , chemistry , enzyme
Flavobacterium meningosepticum produced a peroxidase coordinately with a H 2 O 2 ‐forming nucleoside oxidase under culture conditions where the bacterium inducibly produced the nucleoside oxidase. The nucleoside oxidase and peroxidase both required Fe 2+ or Fe 3+ for their production, and Cu 2+ enhanced the production of both enzymes. The addition of nucleoside analogs to the medium also enhanced the production of both enzymes. The time course of peroxidase production coincided with that of the nucleoside oxidase production. These results suggest that the peroxidase has a specific function related to the nucleoside oxidase reaction, in breaking down the H 2 O 2 formed, which would otherwise cause oxidative cell damage.