Open AccessGenome size and macrorestriction map of Xanthomonas campestris pv. glycines YR32 chromosome
Author(s) -
Widjaja Ricky,
Suwanto Antonius,
Tjahjono Budi
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13602.x
Subject(s) - biology , restriction enzyme , genetics , xanthomonas campestris , chromosome , 23s ribosomal rna , pulsed field gel electrophoresis , restriction map , gene , restriction site , genotype , plasmid , rna , ribosome
Xanthomonas campestris is an important plant pathogenic bacterium which causes severe diseases in a wide variety of plant species. We have generated a macrorestriction map of the X. campestris ( axonopodis ) pv. glycines chromosome employing pulsed‐field gel electrophoresis (PFGE). Restriction endonucleases Pac I (5′‐TTAATTAA), Pme I (5′‐GTTTAAAC) and Swa I (5′‐ATTTAAAT) digested the chromosomal DNA into three, five, and five fragments, respectively. In addition, intron‐encoded restriction endonuclease I‐ Ceu I was employed to locate the position of the 23S rRNA genes ( rrlA and rrlB ). All of the generated restriction fragments were aligned along the chromosome using multiple restriction enzyme digestion and two‐dimensional PFGE (2‐D PFGE) in conjunction with Southern hybridization analysis. This physical map construction has revealed a single circular chromosome with a size of approximately 5 Mb. Two rRNA genes were localized on the chromosome map. Several genes involved in pathogenesis ( xpsD, opsX , and pat ) as well as genes involved in the biosynthesis of xanthan gum ( xanAB, rfbCDAB ) were also localized.