Open AccessA new chloramphenicol and florfenicol resistance gene flanked by two integron structures in Salmonella typhimurium DT104
Author(s) -
Arcangioli MarieAnne,
LeroySétrin Sabine,
Martel JeanLouis,
ChaslusDancla Elisabeth
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13586.x
Subject(s) - integron , biology , genetics , salmonella , florfenicol , gene , microbiology and biotechnology , tetracycline , chloramphenicol , efflux , locus (genetics) , plasmid , bacteria , antibiotics
A new chloramphenicol resistance gene from Salmonella typhimurium DT104, designated floR , also conferring resistance to florfenicol, was characterized. Sequence analysis of the deduced FloR protein suggested that it belongs to the 12‐TMS (transmembrane segments) multidrug efflux pumps family. The floR gene, and the downstream sequenced tetR and tetA tetracycline resistance genes, were surrounded by two class 1 integrons. The first one contained the resistance gene aadA2 and a deleted sulI resistance gene. The second one contained the β‐lactamase gene pse1 and a complete sulI gene. Thus, the floR gene is included in a multiresistance locus of at least 12.5 kb. Its particular organization and chromosomal location could be involved in the antibioresistance pattern stability of the DT104 Salmonella typhimurium strains.