Open AccessLux ‐biosensor assessment of pH effects on microbial sorption and toxicity of chlorophenols
Author(s) -
Sinclair Gillian M,
Paton Graeme I,
Meharg Andy A,
Killham Ken
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13579.x
Subject(s) - pseudomonas fluorescens , adsorption , sorption , biosensor , toxicity , chemistry , escherichia coli , pseudomonas , luminescent bacteria , nuclear chemistry , chromatography , environmental chemistry , bacteria , biology , biochemistry , organic chemistry , genetics , gene
Lux ‐marked bacterial biosensors and a commercial toxicity testing bacterial strain (Microtox™) were exposed to 2,4‐dichlorophenol (DCP) and the light output response measured. Increasing DCP concentrations caused a decrease in light output in all three biosensors with an order of sensitivity (in terms of luminescence decrease over the DCP concentration range) of Pseudomonas fluorescens < Escherichia coli <Microtox™. Adsorption of DCP to E. coli was measured using uniformly ring labelled [ 14 C]DCP and found to be very rapid. The effect of pH on toxicity and adsorption was also investigated. Low pH values increased the amount of DCP adsorbed to the cell and increased the toxicity of DCP.