Open AccessAssembly of the CotSA coat protein into spores requires CotS in Bacillus subtilis
Author(s) -
Takamatsu Hiromu,
Kodama Takeko,
Watabe Kazuhito
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13569.x
Subject(s) - bacillus subtilis , spore , sporangium , mutant , biology , coat , microbiology and biotechnology , biochemistry , bacteria , genetics , gene , paleontology
The CotSA protein, encoded by cotSA ( ytxN ) of Bacillus subtilis , was detected from the cells at 5 h after the onset of sporulation (T 5 ) and in the spore coat of wild‐type cells, but not in cotE, cotS, gerE , or cotSA mutant spores. CotSA was also detected in the sporangium at T 5 to T 7 but not in the sporangium at T 18 of cotS mutant cells, while the incorporation of CotS into the coat was not dependent upon CotSA. These results suggested that CotSA was synthesized simultaneously with CotS during T 5 to T 7 of sporulation and assembled into the coat dependent upon CotS.