Open AccessGenomic organization of the radioresistant bacterium Deinococcus radiodurans : physical map and evidence for multiple replicons
Author(s) -
Kikuchi Masahiro,
Narumi Issay,
Kitayama Shigeru,
Watanabe Hiroshi,
Yamamoto Kazuo
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13562.x
Subject(s) - deinococcus radiodurans , biology , genetics , microbiology and biotechnology , genomic dna , chromosome , replicon , deinococcus , dna , plasmid , genomic organization , gene , genome
A physical map of Deinococcus radiodurans strain KD8301 was constructed by ordering DNA fragments from genomic DNA digested with the enzymes Not I and Pme I. Not I produced 13 fragments and Pme I four fragments. Junction fragments and multiple enzyme digestion of the genomic DNA, in conjunction with Southern hybridization analysis indicated that KD8301 contains chromosome I (2.6 megabases), chromosome II (0.4 megabases) and an endogenous plasmid (0.2 megabases). DNA repair genes such as recA, uvrA, polA and ruvB were found on chromosome I. The lexA gene was on chromosome II. Pulsed‐field gel electrophoresis analysis of undigested DNA indicated that monomer and multimer forms of chromosome II existed in cells. These results suggested that the mechanisms involved in multimer formation may serve as a system for radioresistance of this bacterium.