Open AccessPositively regulated expression of the Escherichia coli araBAD promoter in Corynebacterium glutamicum
Author(s) -
BenSamoun Karine,
Leblon Gérard,
Reyes Oscar
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13558.x
Subject(s) - corynebacterium glutamicum , escherichia coli , rna polymerase , biology , transcription (linguistics) , gene , gene expression , promoter , inducer , corynebacterium , microbiology and biotechnology , bacteria , genetics , linguistics , philosophy
In Corynebacterium glutamicum the promoter of the araBAD Escherichia coli gene is positively regulated by both arabinose and the araC gene product, as it is the case in the natural host. If the l ‐arabinose inducer and an active araC gene are present, significant amounts of araBAD promoter expression take place in the absence of the E. coli CRP protein. These results show that the C. glutamicum RNA polymerase is activated by the E. coli positive regulator of transcription AraC.