Open AccessGrowth requirements of pyruvate‐decarboxylase‐negative Saccharomyces cerevisiae
Author(s) -
Flikweert Marcel T.,
Swaaf Martin,
Dijken Johannes P.,
Pronk Jack T.
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13551.x
Subject(s) - chemostat , pyruvate decarboxylase , saccharomyces cerevisiae , biochemistry , chemistry , chemically defined medium , carboxy lyases , yeast , biology , enzyme , bacteria , alcohol dehydrogenase , in vitro , genetics
Pyruvate‐decarboxylase (Pdc)‐negative Saccharomyces cerevisiae has been reported to grow in batch cultures on glucose‐containing complex media, but not on defined glucose‐containing media. By a combination of batch and chemostat experiments it is demonstrated that even in complex media, Pdc − S. cerevisiae does not exhibit prolonged growth on glucose. Pdc − strains do grow in carbon‐limited cultures on defined media containing glucose‐acetate mixtures. The acetate requirement for glucose‐limited growth, estimated experimentally by continuously decreasing the acetate feed to chemostat cultures, matched the theoretical acetyl‐CoA requirement for lipid and lysine synthesis, consistent with the proposed role of pyruvate decarboxylase in the synthesis of cytosolic acetyl‐CoA.