Open AccessComparison of the electrooptical properties and specific respiratory activity of Acinetobacter calcoaceticum A‐122
Author(s) -
Ignatov Oleg V,
Khorkitaliya A,
Shchyogolev S. Yu. Sergei Yu,
Singirtsev Igor N,
Bunin Victor D,
Tumaikina Yuliya A,
Ignatov Vladimir V
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13538.x
Subject(s) - strain (injury) , chemistry , respiratory system , biophysics , incubation , suspension (topology) , respiration , metabolism , biochemistry , biology , botany , mathematics , anatomy , homotopy , pure mathematics
The electrooptical (EO) properties of a cell suspension and the specific respiratory activity of cells towards p ‐nitrophenol (PNP) were compared during PNP metabolism in Acinetobacter calcoaceticum strain A‐122. The frequency dependence of the suspension's turbidity changes due to cellular orientation (orientational spectra) at frequencies of an orienting electric field of 10–10 000 kHz was determined. Orientational spectral changes observed during PNP incubation of the cells were followed over the range of 10–502 kHz. There were linear relationships between the magnitude of the EO effect at a 502‐kHz frequency and the concentration of PNP over the range of 0.1–0.8 mM, and between the specific respiratory activity of the cells and the concentration of PNP over the range of 0.1–1.0 mM. The knowledge gained from these studies suggests a direct relationship between alterations in the cellular EO properties and PNP metabolism.