Open AccessNitrogen regulation in Corynebacterium glutamicum : isolation of genes involved and biochemical characterization of corresponding proteins
Author(s) -
Jakoby Marc,
Krämer Reinhard,
Burkovski Andreas
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13518.x
Subject(s) - corynebacterium glutamicum , biochemistry , biology , glutamine synthetase , adenylylation , escherichia coli , gene , stringent response , mutagenesis , glutamine amidotransferase , mutant , glutamine , biosynthesis , amino acid
The regulation of nitrogen assimilation was investigated in the Gram‐positive actinomycete Corynebacterium glutamicum . Biochemical studies and site‐directed mutagenesis revealed that glutamine synthetase activity is regulated via adenylylation in this organism. The genes encoding the central signal transduction protein P II ( glnB ) and the primary nitrogen sensor uridylyltransferase ( glnD ) were isolated and sequenced. Additionally, genes putatively involved in the degradation of ornithine ( ocd ) and sarcosine ( soxA ), ammonium uptake ( amtP ) and protein secretion ( ftsY, srp ) were identified in C. glutamicum . Based on these observations, the mechanism of N regulation in C. glutamicum is similar to that of the Gram‐negative Escherichia coli . As deduced from data base searches, the described regulation may also hold true for the important pathogen Mycobacterium glutamicum .