Open AccessCo‐existence of clpB and clpC in the Bacillaceae
Author(s) -
Namy Olivier,
Mock Michèle,
Fouet Agnès
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13517.x
Subject(s) - clpb , bacillus subtilis , biology , bacillus anthracis , gene , microbiology and biotechnology , genetics , bacteria , escherichia coli
The gene encoding ClpC in Bacillus anthracis was amplified from the chromosome by polymerase chain reaction using degenerate oligonucleotide primers. These primers also amplified a second DNA fragment identified as a clpB homolog. Both genes were suggested to be functional. Contrary to Bacillus subtilis which possesses clpC but not clpB , many Bacillus species were found to harbor both clpC and clpB . We also found that Clostridium strains could possess clpB, clpC , or both. All the Gram‐negative strains tested had clpB only.