Open AccessA new pair of primers designed for amplification of the ITS region in Tuber species
Author(s) -
Bertini Luana,
Amicucci Antonella,
Agostini Deborah,
Polidori Emanuela,
Potenza Lucia,
Guidi Chiara,
Stocchi Vilberto
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13508.x
Subject(s) - internal transcribed spacer , truffle , spacer dna , ribosomal dna , biology , genetics , gene , ribosomal rna , polymerase chain reaction , microbiology and biotechnology , botany , phylogenetics
The alignment of the 28S gene of several species of Pezizales allowed to select two pairs of primers able to amplify the internal transcribed spacer region of ribosomal DNA in mycorrhizal fungi, such as truffles. The higher yield of the amplification product demonstrates a better annealing of the new primers to the rDNA, as compared to the universal primers internal transcribed spacer 1 and internal transcribed spacer 4. Therefore, the new primers can be used as an easier and more sensitive tool for the identification of truffle species in any stage of their life cycle, including the mycorrhizal phase.