Open AccessMicroscopic methods for distinguishing among three cell types in TOL plasmid‐carrying Pseudomonas putida cultures
Author(s) -
Lu YunnTzer,
Love Nancy G,
Grady C.P.Leslie
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13502.x
Subject(s) - plasmid , agarose , chemostat , biology , pseudomonas putida , carbenicillin , microbiology and biotechnology , chemistry , gene , genetics , bacteria , ampicillin
Microscopic methods were developed that enable the sensitive quantification of different cell types that are generated by plasmid instability processes when Pseudomonas putida PaW164 (X + ), which carries a TOL plasmid (pWW0‐164), is grown in chemostat culture. Cells that have lost the structural TOL genes (X − ) or the entire TOL plasmid (X 0 ) can be quantified in a background of 6000 X + cells using catechol agarose miniplates. X 0 cells can be quantified in a background of 3500 X + or X − cells using carbenicillin agarose miniplates. These methods represent significant improvements in sensitivity over conventional plating methods.