Open AccessFunctional analysis of the hemK gene product involvement in protoporphyrinogen oxidase activity in yeast
Author(s) -
Le Guen Laurence,
Santos Renata,
Camadro JeanMichel
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13499.x
Subject(s) - protoporphyrinogen oxidase , complementation , yeast , biochemistry , saccharomyces cerevisiae , biology , escherichia coli , gene , microbiology and biotechnology , phenotype
The Escherichia coli hemK gene has been described as being involved in protoporphyrinogen oxidase activity; however, there is no biochemical evidence for this. In the context of characterizing the mechanisms of protoporphyrinogen oxidation in the yeast Saccharomyces cerevisiae , we investigated the yeast homolog of HemK, which is encoded by the ORF YNL063w, to find out whether it has any protoporphyrinogen oxidase activity and/or whether it modulates protoporphyrinogen oxidase activity. Phenotype analysis and enzyme activity measurements indicated that the yeast HemK homolog is not involved in protoporphyrinogen oxidase activity. Complementation assays in which the yeast HemK homolog is overproduced do not restore wild‐type phenotypes in a yeast strain with deficient protoporphyrinogen oxidase activity. Protein sequence analysis of HemK‐related proteins revealed consensus motif for S ‐adenosyl‐methionine‐dependent methyltransferase.