Open AccessHigh‐resolution genomic fingerprinting of Campylobacter jejuni and Campylobacter coli by analysis of amplified fragment length polymorphisms
Author(s) -
Kokotovic Branko,
On Stephen L.W
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13487.x
Subject(s) - campylobacter jejuni , campylobacter , biology , campylobacter coli , dna profiling , genetics , microbiology and biotechnology , restriction fragment length polymorphism , polymorphism (computer science) , genotype , bacteria , gene , dna
A method for high‐resolution genomic fingerprinting of the enteric pathogens Campylobacter jejuni and Campylobacter coli , based on the determination of amplified fragment length polymorphism, is described. The potential of this method for molecular epidemiological studies of these species is evaluated with 50 type, reference, and well‐characterised field strains. Amplified fragment length polymorphism fingerprints comprised over 60 bands detected in the size range 35–500 bp. Groups of outbreak strains, replicate subcultures, and ‘genetically identical’ strains from humans, poultry and cattle, proved indistinguishable by amplified fragment length polymorphism fingerprinting, but were differentiated from unrelated isolates. Previously unknown relationships between three hippurate‐negative C. jejuni strains, and two C. coli var. hyoilei strains, were identified. These relationships corresponded to available epidemiological data. We conclude that this amplified fragment length polymorphism fingerprinting method may be a highly effective tool for molecular epidemiological studies of Campylobacter spp.