Open AccessMolecular cloning and phylogenetic analysis of the small cytoplasmic RNA from Listeria monocytogenes
Author(s) -
Barry Tom,
Kelly Mary,
Glynn Barry,
Peden John
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13483.x
Subject(s) - biology , listeria monocytogenes , cloning (programming) , recombinant dna , clone (java method) , complementation , rna , gene , bacteria , molecular cloning , listeria , protein fragment complementation assay , microbiology and biotechnology , genetics , gene expression , computer science , phenotype , programming language
A molecular cloning strategy has been designed to isolate the gene that encodes the small cytoplasmic RNA (scRNA) component of bacterial signal recognition particles. Using this strategy a putative Listeria monocytogenes scRNA λgt11 recombinant clone was isolated. A previously described complementation assay developed to genetically select functional homologues of 4.5S RNA and scRNA of bacteria confirmed that the λgt11 recombinant clone isolated encoded for the scRNA from L. monocytogenes . A secondary structure for this scRNA is proposed and a phylogenetic comparison of the 276 base L. monocytogenes scRNA with previously characterised Gram‐positive bacterial scRNAs is also presented.