Open AccessCharacterisation of kinetoplast DNA minicircles from Herpetomonas samuelpessoai
Author(s) -
Fu Guoliang,
Lambson Bronwen,
Barker Douglas
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13451.x
Subject(s) - minicircle , biology , kinetoplast , genetics , dna , gene , sequence (biology) , sequence analysis , dna sequencing , conserved sequence , computational biology , base sequence
In this study, we have sequenced more than 100 clones of minicircle DNA from Herpetomonas samuelpessoai . An unusual amplification approach was developed to amplify minicircle DNA by using a pair of complementary primers designed from a universal stretch of minicircle sequence. Sequence analysis shows that the kinetoplast minicircles in Herpetomonas with a size of 1.3 kb are organised into two conserved regions and two variable regions which are located 180° apart. The potential gRNA genes are encoded in variable regions of minicircle approximately 360 bp from CSB‐3 (conserved sequence block 3). A conserved upstream sequence located 30 nt before the gRNA genes was identified and is related to the gRNA genes in sequence organisation. A potential role(s) of this sequence in gRNA transcription is discussed.