Open AccessPhylogenetic relationships between toxic and non‐toxic strains of the genus Microcystis based on 16S to 23S internal transcribed spacer sequence
Author(s) -
Otsuka Shigeto,
Suda Shoichiro,
Li Renhui,
Watanabe Masayuki,
Oyaizu Hiroshi,
Matsumoto Satoshi,
Watanabe Makoto M
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13443.x
Subject(s) - internal transcribed spacer , spacer dna , phylogenetic tree , biology , microcystis , ribosomal dna , intergenic region , ribosomal rna , phylogenetics , botany , 16s ribosomal rna , genus , genetics , gene , bacteria , cyanobacteria , genome
16S to 23S ribosomal DNA internal transcribed spacer sequences of 47 strains of the genus Microcystis were determined. Derived maximum likelihood and DNA distance trees indicated that Microcystis can be divided into three clusters. The first cluster included toxic and non‐toxic strains, the second only toxic ones, and the third only non‐toxic ones. The tree topologies were not necessarily correlated with morphospecies distinction or phycobilin pigment composition, and one genotype may have more than one morphotype. Phylogenetic analysis based on intergenic spacer sequences was thought to be effective for understanding relationships among closely related species and strains.