Open AccessInfluence of manganese on cell morphology, protoplasts formation and β‐ d ‐glucosidase activity in Phaffia rhodozyma
Author(s) -
Pera Licia M,
Rubinstein Laura,
Baigorı́ Mario D,
Figueroa Lucı́a I.C,
Callieri Danley A
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13426.x
Subject(s) - protoplast , yeast , cell wall , enzyme , manganese , chemistry , cellulase , biochemistry , stabiliser , enzyme assay , biology , food science , organic chemistry
Among several potential cell wall lytic enzymes, such as β‐ d ‐glucosidase, β‐ d ‐galactosidase, β‐ N ‐acetyl‐ d ‐glucosaminidase, β‐ N ‐acetyl‐ d ‐galactosaminidase, and β‐ d ‐ N,N ′‐diacetylchitinase, Phaffia rhodozyma showed only β‐ d ‐glucosidase activity. MnCl 2 increased the activity, but not the production of the enzyme. When the yeast was grown with 4.0 mM MnCl 2 , the cells were bigger and rounder than those obtained in its absence, suggesting a weakening of the cell wall. Protoplasts were obtained from these cells, using 0.3 M KCl as the osmotic stabiliser.