Open AccessInduction of humoral and cellular immune responses in mice by a recombinant fowlpox virus expressing the E2 protein of bovine viral diarrhea virus
Author(s) -
Mehdy Elahi Seyyed,
Bergeron Jean,
Nagy Éva,
Talbot Brian Geoffrey,
Harpin Serge,
Shen ShiHsiang,
Elazhary Youssef
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13419.x
Subject(s) - fowlpox virus , virology , virus , biology , immune system , recombinant dna , humoral immunity , antibody , recombinant virus , interferon , microbiology and biotechnology , immunology , gene , biochemistry
A recombinant fowlpox virus (rFPV/E2) expressing the E2 protein of bovine viral diarrhea virus (BVDV) was constructed and characterized. Mice were immunized with recombinant virus and both humoral and cellular immune responses were studied. rFPV/E2 induced BVDV‐specific antibodies which were detected by ELISA. In addition, mouse sera were shown to neutralize BVDV. A cytokine ELISA assay revealed that mice vaccinated with rFPV/E2 induced 7‐fold more interferon‐γ than parental fowlpox virus.