Open AccessAcquisition of aluminum tolerance in Saccharomyces cerevisiae by expression of the BCB or NtGDI1 gene derived from plants
Author(s) -
Ezaki Bunichi,
Sivaguru Mayandi,
Ezaki Yuka,
Matsumoto Hideaki,
Gardner Richard C
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13415.x
Subject(s) - arabidopsis , saccharomyces cerevisiae , gene , gene expression , gene product , biology , chemistry , biochemistry , genetics , microbiology and biotechnology , mutant
Eleven aluminum stress‐induced genes derived from plants (wheat, Arabidopsis and tobacco) were introduced into Saccharomyces cerevisiae to test if expression of these genes confers Al tolerance. Al sensitivity tests showed that expression of two genes, either an Arabidopsis gene for blue copper binding protein ( BCB ), or a tobacco gene for the GDP dissociation inhibitor ( NtGDI1 ), conferred Al tolerance. Determinations of total content and localization of Al ions in these transformants suggested that the BCB gene product functions in restricting Al uptake, while expression of the NtGDI1 gene promotes release of Al ions after uptake.