Open AccessThe mitochondrial genome of Mucor piriformis
Author(s) -
Papp Tamás,
Palágyi Zsuzsanna,
Ferenczy Lajos,
Vágvölgyi Csaba
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13413.x
Subject(s) - mitochondrial dna , biology , restriction map , dna , genome , aspergillus nidulans , genetics , restriction enzyme , microbiology and biotechnology , gene , plasmid , mutant
DNA was purified from the isolated mitochondria of a Mucor piriformis wild‐type strain (NRRL 26211). A circular restriction map of the mitochondrial DNA was established on the basis of single and double digests with several restriction endonucleases. The average mitochondrial DNA size calculated from these data was 33.53 kbp; this is in good agreement with the contour length size (33.62 kbp) of the open circular molecules detected by electron microscopy. Heterologous hybridizations with cloned Aspergillus nidulans mitochondrial genes were used to locate some coding regions on the map.