Open AccessToxin‐co‐regulated pilus cluster in non‐O1, non‐toxigenic Vibrio cholerae : evidence of a third allele of pilin gene
Author(s) -
Novais Rogério C,
Coelho Ana,
Salles Carlos A,
Vicente Ana C.P
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13411.x
Subject(s) - pilin , vibrio cholerae , pilus , biology , gene cluster , genetics , gene , restriction fragment length polymorphism , nucleic acid sequence , microbiology and biotechnology , toxin , polymerase chain reaction , virulence , bacteria
Abstract Polymerase chain reaction has been used to detect the presence of the virulence associated gene, tcpA and part of the promoter distal region of the toxin‐co‐regulated pilus cluster in non‐O1, non‐toxigenic, Vibrio cholerae . The amplified regions were characterised by restriction fragment length polymorphism and heteroduplex motility assay. We describe the nucleotide sequence of the tcpA gene fragment from non‐toxigenic vibrios from clinical and environmental sources. The present study shows that there are at least three types of the tcpA gene among V. cholerae and the primers specific for the classical tcpA gene, amplify all biotypes. A sequence similarity in other regions of the toxin‐co‐regulated pilus cluster is suggested. The evidences for the presence of this cluster among non‐toxigenic vibrios is, to our knowledge, reported for the first time. The use of restriction fragment length polymorphism for typing the tcpA and studying the alleles distribution is proposed.