Open AccessA consensus sequence for the Rhodospirillaceae SOS operators
Author(s) -
Labazi Mohamed,
Rey Alfonso,
Fernandez de Henestrosa Antonio R,
Barbé Jordi
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13409.x
Subject(s) - rhodobacter , rhodobacter sphaeroides , rhodospirillaceae , rhodospirillales , biology , promoter , gene , consensus sequence , genetics , rhodopseudomonas palustris , dna , peptide sequence , gene expression , bacteria , mutant
The sequences controlling the expression of the Rhodobacter capsulatus recA and uvrA genes belonging to the SOS DNA repair system have been identified by PCR mutagenesis. Data obtained demonstrated that the GTTCN 7 GTAC and GAACN 7 GAAC motifs present upstream of the recA gene and the GTTCN 7 GTTC motif found upstream of the uvrA gene are required for their respective DNA damage‐mediated induction. Alignment of recA promoters of R. capsulatus, Rhodobacter sphaeroides and Rhodopseudomonas viridis with the uvrA promoters of R. capsulatus and R. sphaeroides has identified the consensus sequence GTTCVYVYTWTGTTC as the SOS operator site of the Rhodospirillaceae family.