Open AccessIdentification and characterisation of IS 1383 , a new insertion sequence isolated from Pseudomonas putida strain H
Author(s) -
Lauf Undine,
Müller Christian,
Herrmann Heidrun
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13401.x
Subject(s) - insertion sequence , inverted repeat , genetics , pseudomonas putida , biology , plasmid , direct repeat , transposase , nucleic acid sequence , homology (biology) , transposable element , sequence analysis , peptide sequence , microbiology and biotechnology , dna , amino acid , gene , base sequence , genome
A new insertion sequence (IS 1383 ) was identified on plasmids from Pseudomonas putida strain H and its nucleotide sequence was determined. IS 1383 contains perfect terminal inverted repeats of 13‐bp flanking a 1.4‐kb internal sequence. A single significant open reading frame was identified that can encode a 342‐amino acid polypeptide which was predicted to be highly basic and to have homology to polypeptides known from several other bacterial insertion sequences. At least six copies of IS 1383 are present on the plasmids pPGH1 and pPGH2, whereas no copy could be detected on the chromosome of P. putida strain H. Target duplications did not flank the inverted repeats of any of the six IS 1383 copies examined. Analysis of the integration sites of IS 1383 revealed hints for a target specificity. Multiple sequence alignments of the transposases, the inverted repeats and the integration sites pointed to the assignment of IS 1383 into a putative new family of insertion sequences defined as the IS 1111 family.