Open Access2,4‐(Hydroxyphenyl)‐ethanol, an antioxidative agent produced by Candida spp., impairs neutrophilic yeast killing in vitro
Author(s) -
Cremer Josef,
Vatou Vassilia,
Braveny Ilja
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13390.x
Subject(s) - candida albicans , zymosan , corpus albicans , microbiology and biotechnology , chemiluminescence , candida parapsilosis , in vitro , ic50 , biology , flow cytometry , chemistry , biochemistry , chromatography
Culture supernatants of Candida albicans were examined for factors with inhibitory activity against the chemiluminescence of human neutrophils. By high resolution gel chromatography, a low‐molecular‐mass chemiluminescence inhibitor was isolated. The compound was identified as 2,4‐(hydroxyphenyl)‐ethanol. Half‐maximum inhibition (IC 50 ) of the chemiluminescence response of neutrophils phagocytizing opsonized zymosan or C. albicans occurred at 38.1±2.3 μM and 19.9±8.3 μM, respectively. As shown by flow cytometry, the compound protected C. albicans against phagocytic killing (IC 50 =73.8±16.9 μM). Substantially higher concentrations of the inhibitor were produced by C. albicans and C. tropicalis than by C. parapsilosis and C. glabrata , suggesting a potential role in pathogenicity ranking.