Open AccessViable, but non‐culturable, state of a green fluorescence protein‐tagged environmental isolate of Salmonella typhi in groundwater and pond water
Author(s) -
Cho JangCheon,
Kim SangJong
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13382.x
Subject(s) - salmonella typhi , green fluorescent protein , microcosm , microbiology and biotechnology , biology , salmonella , enterobacteriaceae , bacteria , gene , escherichia coli , ecology , biochemistry , genetics
An environmental isolate of Salmonella typhi was chromosomally marked with a gfp gene encoding green fluorescence protein (GFP) isolated from Aequorea victoria . The hybrid transposon mini‐Tn5 gfp was transconjugated from E . coli to S . typhi , resulting in constitutive GFP production. The survival of S . typhi GFP155 introduced into groundwater and pond water microcosms was examined by GFP‐based plate counts, total cell counts, and direct viable counts. A comparison between GFP‐based direct viable counts and plate counts was a good method for verifying the viable, but non‐culturable (VBNC), state of S . typhi . The entry into a VBNC state of S . typhi was shown in all microcosms. S . typhi survived longer in groundwater than in pond water as both a culturable and a VBNC state.