Open AccessReversible inactivation of nitrogenase in Rhodobacter capsulatus strain W107I deleted in the draTG gene region
Author(s) -
Förster Beate,
Maner Klaus,
Fassbinder Frank,
Oelze Jürgen
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13370.x
Subject(s) - rhodobacter , nitrogenase , mutant , biochemistry , strain (injury) , gene , biology , enzyme , rhodospirillaceae , darkness , chemistry , genetics , bacteria , nitrogen fixation , botany , anatomy
Upon addition of ammonium or removal of light, nitrogenase was reversibly inactivated in a draTG gene deletion mutant of Rhodobacter capsulatus . Reversible inactivation by exposure of the mutant to darkness followed the same kinetics as in control strains containing the draTG gene region. Reactivation of nitrogenase by light was significantly faster than removal of ADP‐ribose from component 2 of nitrogenase. The results show that, in R . capsulatus , reversible inactivation of nitrogenase is independent of reversible ADP‐ribosylation by the DRAT/DRAG enzyme system encoded by the draTG gene region.