Open AccessIsolation of csm1 encoding a class V chitin synthase with a myosin motor‐like domain from the rice blast fungus, Pyricularia oryzae
Author(s) -
Park In Cheol,
Horiuchi Hiroyuki,
Hwang Cher Won,
Yeh Wan Hae,
Ohta Akinori,
Ryu Jin Chang,
Takagi Masamichi
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13365.x
Subject(s) - chitin synthase , chitin , biology , aspergillus nidulans , atp synthase , genetics , gene , pyricularia , microbiology and biotechnology , biochemistry , botany , chitosan , mutant
A gene encoding a chitin synthase with a myosin motor‐like domain ( csm1 ) was isolated from Pyricularia oryzae using a PCR fragment amplified from a fungal chitin synthase conserved region. The deduced amino acid sequence of csm1 is homologous to that of CsmA of Aspergillus nidulans (65% identity). The putative gene product of csm1 is consisted of the myosin motor‐like domain and a chitin synthase domain as in A . nidulans csmA . The chitin synthase domain of its C‐terminus was also homologous to Aspergillus fumigatus ChsE (61.4% identity) and Ustilago maydis Chs6 (48.6% identity) that encode class V chitin synthases. Northern analysis demonstrated that the csm1 was expressed throughout the mycelial growth of P . oryzae . This is the first report on the isolation of the gene encoding a class V chitin synthase with the myosin motor‐like domain from P . oryzae .