Open AccessInduction and enhancement of stress proteins in a trichloroethylene‐degrading methanotrophic bacterium, Methylocystis sp. M
Author(s) -
Uchiyama Hiroo,
Shinohara Yuko,
Tomioka Noriko,
Kusakabe Isao
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13364.x
Subject(s) - trichloroethylene , heat shock protein , bacteria , chemistry , gel electrophoresis , environmental chemistry , biochemistry , chromatography , biology , gene , genetics
The responses of the trichloroethylene‐degrading bacterium Methylocystis sp. M to six different water‐pollutants, carbon starvation, and temperature‐shock (heat and cold) were examined using 2‐dimensional gel electrophoresis. Twenty‐eight polypeptides were induced, and these stress‐induced proteins were classified into three groups. Some of the chemically induced proteins were the same as those induced by carbon starvation and temperature‐shock. Two of the polypeptides were induced by trichloroethylene. Trichloroethylene‐stress protein synthesis required 1–2 h at a concentration of trichloroethylene that had no effect on growth. Furthermore, 25 stress‐enhanced polypeptides were observed, and one of these was enhanced by trichloroethylene. Based on these results, we discuss applications of chemical‐stress induction of proteins to establish effective bioremediation and bioassay by methanotrophs.