Open AccessTranscriptional regulation of Nostoc uptake hydrogenase
Author(s) -
Axelsson Rikard,
Oxelfelt Fredrik,
Lindblad Peter
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13357.x
Subject(s) - nostoc , heterocyst , cyanobacteria , anabaena variabilis , hydrogenase , enzyme , biology , biochemistry , strain (injury) , anabaena , microbiology and biotechnology , nitrogenase , nitrogen fixation , bacteria , genetics , anatomy
Filamentous, heterocystous cyanobacteria may contain both an uptake hydrogenase (encoded by hupSL ) and a bidirectional enzyme (encoded by hoxFUYH ). The present study identifies three strains ( Anabaena variabilis, Nostoc muscorum and Nostoc sp. strain PCC 73102) with a contiguous hupL in both vegetative cells and heterocysts. The two Nostoc strains differ in either containing a bidirectional enzyme ( N . muscorum ) or lacking this enzyme ( N . PCC 73102). Transcriptional studies, using reverse transcriptase‐polymerase chain reaction, demonstrated an induction of a hupL transcript approximately 24 h after a shift from non‐nitrogen‐fixing to nitrogen‐fixing conditions (in parallel with the induction of an in vivo light‐dependent H 2 ‐uptake activity) in N . muscorum . However, the level of hoxH transcripts did not change significantly during the induction of the H 2 ‐uptake activity.