Open AccessVariable expression of glutathione S ‐transferase isoenzymes in the fungus, Mucor circinelloides
Author(s) -
Dowd Caitriona A,
Sheehan David
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb13349.x
Subject(s) - mucor circinelloides , glutathione , glutathione s transferase , isozyme , biology , biochemistry , transferase , enzyme , protein subunit , microbiology and biotechnology , mucor , genetics , gene , penicillium
Mucor circinelloides (previously M . javanicus ) variably expresses two isoenzymes of glutathione S ‐transferases. Glutathione S ‐transferases 1 and 2 were purified by affinity chromatography and found to be dimers with subunit M r values of 25.5 and 28. While glutathione S ‐transferase 1 immunoblotted with rat glutathione S ‐transferase T 5‐5, N‐terminal sequencing indicated that this enzyme was distinct from recently characterised bacterial and fungal glutathione S ‐transferases and did not readily fit into any one class. It showed some similarity to several classes including insect class II and plant type III. M . circinelloides glutathione S ‐transferase 2 did not immunoblot and yielded no N‐terminal sequence.