Open AccessAnalysis of genetic diversity at the iron‐containing superoxide dismutase locus in Plasmodium falciparum wild isolates
Author(s) -
Baert Céline Bruzi,
Deloron Philippe,
Viscogliosi Eric,
Dauchez Manuel,
Camus Daniel,
Dive Daniel
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08850.x
Subject(s) - biology , plasmodium falciparum , superoxide dismutase , gene , locus (genetics) , genetic diversity , genetics , enzyme , microbiology and biotechnology , biochemistry , malaria , immunology , population , demography , sociology
In order to investigate the genetic diversity of iron‐containing superoxide dismutase (FeSOD) from Plasmodium falciparum , a potential anti‐malarial therapeutic target, we cloned and sequenced Plasmodium FeSOD from 26 blood samples from non‐infected patients. Fifteen clones had the same nucleotide sequence as that of the FeSOD gene of the P. falciparum strain HB3 cultivated in vitro. The other 11 clones presented mutations responsible for punctual amino acid changes which did not modify key residues for the function or the structure of the enzyme. The high sequence conservation between FeSOD from the isolates confirms that this enzyme could represent a therapeutic target.