Open AccessPresence of non‐supressive, M2‐related dsRNAs molecules in Saccharomyces cerevisiae strains isolated from spontaneous fermentations
Author(s) -
Cansado José,
Barros Velázquez Jorge,
Sieiro Carmen,
Gacto Mariano,
Villa Tomás G.
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08846.x
Subject(s) - rna silencing , saccharomyces cerevisiae , biology , strain (injury) , rna , microbiology and biotechnology , virology , genetics , gene , rna interference , anatomy
Total dsRNA extractions in five killer K2 strains of Saccharomyces cerevisiae isolated from spontaneous fermentations revealed the presence of a novel dsRNA fragment (which we named NS dsRNA) of approximately 1.30 kb, together with L and M2 dsRNAs. NS dsRNA appeared to be encapsidated in the same kind of viral particles as L and M2 dsRNA. Northern blot hybridization experiments indicated that NS dsRNA was derived from M2 dsRNA, likely by deletion of the internal A+U‐rich region. However, unlike S dsRNAs (suppressive forms derived from M1 dsRNA in K1 killers), NS dsRNA did not induce exclusion of the parental M2 dsRNA when the host strain was maintained for up to 180 generations of growth.