Open AccessGrowth and cytotoxic activity by Mycobacterium ulcerans in protein‐free media
Author(s) -
MveObiang Armand,
Remacle José,
Palomino Juan Carlos,
Houbion Andrée,
Portaels Françoise
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08838.x
Subject(s) - mycobacterium ulcerans , buruli ulcer , virulence , microbiology and biotechnology , virulence factor , organism , toxin , biology , cytotoxic t cell , disease , medicine , biochemistry , pathology , genetics , in vitro , gene
The pathogenic slow‐growing Mycobacterium ulcerans has, until now, been cultured in liquid media containing albumin. Here, we report the first description of use of Sauton medium and modified Reid medium, two protein‐free media, in which M. ulcerans was able to grow and produce its toxin, a major virulence factor of this environmental organism which causes a skin disease commonly called Buruli ulcer. These results suggest that Sauton and modified Reid may be useful for certain fields of M. ulcerans research requiring protein‐free growth conditions.