Open AccessSynthesis of chitinase in Streptomyces thermoviolaceus is regulated by a two‐component sensor‐regulator system
Author(s) -
Tsujibo Hiroshi,
Hatano Naoya,
Okamoto Takashi,
Endo Hiroshi,
Miyamoto Katsushiro,
Inamori Yoshihiko
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08829.x
Subject(s) - chitinase , gene , streptomyces , biology , response regulator , histidine , amino acid , locus (genetics) , two component regulatory system , extracellular , genetics , subfamily , biochemistry , bacteria , bacterial protein , mutant
The chiS and chiR genes located upstream of the chitinase locus ( chi40 ) on the chromosome of Streptomyces thermoviolaceus OPC‐520 were cloned and sequenced. The deduced amino acid sequences revealed that ChiS (390 amino acids, 40.9 kDa) and ChiR (213 amino acids, 22 kDa) show significant sequence similarities to histidine kinases and response regulators, respectively, of typical prokaryotic two‐component regulatory systems. The extracellular chitinase activity of Streptomyces lividans 66 (pTSR2 (bearing chiS , chiR and chi40 )) was significantly enhanced by a high dosage of the chiS and chiR genes.