Open AccessIsolation and characterisation of the lipopolysaccharide from Xanthomonas hortorum pv. vitians
Author(s) -
Molinaro Antonio,
Lanzetta Rosa,
Evidente Antonio,
Parrilli Michelangelo,
Holst Otto
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08825.x
Subject(s) - rhamnose , lipid a , xanthomonas , fucose , mannose , biochemistry , lipopolysaccharide , fatty acid , bacteria , glucosamine , polysaccharide , galactose , chemistry , biology , microbiology and biotechnology , genetics , gene , endocrinology
Xanthomonas hortorum pv. vitians is a Gram‐negative bacterium that acts as the causative agent of bacterial leaf spot and headrot in lettuce. The lipopolysaccharide (LPS) of this bacterium is suspected to be an important molecule for adhesion to the plants. We have isolated the LPS, prepared the lipid A and the polysaccharide moieties thereof, and characterised all preparations by compositional analysis. Main sugar components are rhamnose and 3‐acetamido‐3,6‐dideoxy‐galactose which presumably furnish the O ‐specific polysaccharide. Other sugars are mannose, glucose, 6‐deoxygalactose (fucose), and galacturonic acid, which should be core region constituents, and glucosamine, which builds up the carbohydrate backbone of lipid A. The LPS contains several phosphate groups, most of which are present in the core region. The main fatty acids in the lipid A are C10:0, 3‐OH‐C10:0 and 3‐OH‐C12:0. The latter is the only amide‐linked fatty acid. Two fatty acids present in small amounts were identified, C8:0 and C11:0.