Open AccessA small cryptic plasmid from Ruminobacter amylophilus NIAH‐3 possesses functional mobilization properties
Author(s) -
Ogata Koretsugu,
Sekizaki Tsutomu,
Aminov Roustam I.,
Tajima Kiyoshi,
Nakamura Mutsumi,
Nagamine Takafumi,
Matsui Hiroki,
Benno Yoshimi
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08824.x
Subject(s) - orfs , plasmid , biology , escherichia coli , bacteria , genetics , amino acid , nucleic acid sequence , dna , peptide sequence , open reading frame , gene
The complete nucleotide sequence of a small cryptic plasmid designated pRAO1, from the Gram‐negative ruminal bacterium Ruminobacter amylophilus NIAH‐3, was determined. The plasmid is a circular DNA molecule, 2140 bp in size, with a GC content of 40%. Computer‐assisted analysis identified three open reading frames (ORFs), one of which, ORF3 (347 amino acids), displayed a high degree of amino acid identity with the Mob proteins involved in conjugative mobilization and interplasmid recombination of plasmids from Gram‐positive bacteria. We proved the mobilization properties of pRAO1 in the Escherichia coli system using the coresident IncW broad‐host‐range conjugative plasmid R388. These data demonstrated, for the first time, the mobilization properties of small cryptic plasmids from Gram‐negative inhabitants of the rumen.