Open AccessGenetic rearrangements in the tyrB ‐ uvrA region of the enterobacterial chromosome: a potential cause for different class B acid phosphatase regulation in Salmonella enterica and Escherichia coli
Author(s) -
Thaller Maria Cristina,
Schippa Serena,
Bonci Alessandra,
Berlutti Francesca,
Selan Laura,
Rossolini Gian Maria
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08821.x
Subject(s) - escherichia coli , salmonella enterica , biology , gene , locus (genetics) , genetics , phosphatase , microbiology and biotechnology , biochemistry , enzyme
Unlike in Escherichia coli , in Salmonella enterica production of class B acid phosphatase (AphA) was detectable also in cells growing in the presence of glucose. Characterization of the aphA locus from a S. enterica ser. typhi strain showed that the aphA determinant is very similar to the E. coli homolog, and that its chromosomal location between the highly conserved tyrB and uvrA genes is retained. However, the aphA flanking regions were found to be markedly different in the two species, either between tyrB and aphA or between aphA and uvrA . The differences in the aphA 5′‐flanking region, which in S. enterica is considerably shorter than in E. coli (183 vs. 1121 bp) and includes potential promoter sequences not present in E. coli , could be responsible for the different regulation of class B acid phosphatase observed in the two species.