Open AccessDouble‐stranded RNA and virus‐like particles in the edible basidiomycete Flammulina velutipes (Enokitake)
Author(s) -
Magae Yumi,
Hayashi Noriko
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08814.x
Subject(s) - flammulina , rna silencing , strain (injury) , mycelium , rna , cytoplasm , virus , biology , virus like particle , chemistry , microbiology and biotechnology , virology , biochemistry , gene , botany , mushroom , rna interference , anatomy , recombinant dna
One of the commercial strains of Flammulina velutipes was analyzed for the presence of double‐stranded RNA (dsRNA) elements to examine the underlying mechanism of strain degeneration. As a result, two dsRNA elements sized 1.9 and 1.8 kb were detected in mycelium derived from spontaneously brown‐colored fruit body. They were not detected in the normal strains or in fruiting‐impaired degenerative isolates. The dsRNAs were not in the nuclear or mitochondrial fractions, but were located in the cytoplasmic fraction. The presence of virus‐like particles of ca. 50 nm diameter associated with the dsRNAs was confirmed by electron microscopic observation.