Open AccessIdentification of the syntrophic partners in a coculture coupling anaerobic methanol oxidation to Fe(III) reduction
Author(s) -
Daniel Rolf,
Warnecke Falk,
Potekhina Joanna S,
Gottschalk Gerhard
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08796.x
Subject(s) - shewanella putrefaciens , methanol , chemistry , anaerobic oxidation of methane , redox , anaerobic exercise , methane , shewanella , microbiology and biotechnology , biochemistry , bacteria , inorganic chemistry , catalysis , biology , organic chemistry , physiology , genetics
From enrichments with methanol and ferric pyrophosphate a coculture was isolated which coupled methanol oxidation to carbon dioxide with the reduction of Fe(III) to Fe(II). 16S rRNA gene analysis of the isolated syntrophic partners revealed 99.5% similarity to Clostridium sphenoides and 98.5% to Shewanella putrefaciens . Formation of Fe(II) coupled to methanol oxidation was confirmed by using strains of culture collections ( C. sphenoides DSM 632 and S. putrefaciens DSM 9461). The importance of this process is discussed, also with respect to the anaerobic oxidation of methane.