Open AccessEffect of tyrosinase inhibitors on Tuber borchii mycelium growth in vitro
Author(s) -
Poma Anna,
Pacioni Giovanni,
Colafarina Sabrina,
Miranda Michele
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08779.x
Subject(s) - tropolone , mycelium , tyrosinase , kojic acid , chemistry , in vitro , biochemistry , biology , botany , enzyme , organic chemistry
This paper reports the effect of the tyrosinase (monophenol o ‐diphenol:oxygen oxidoreductase; EC 1.14.18.1) inhibitors diethyldithiocarbamate (DETC), L ‐tropolone, kojic acid, phenylthiourea (PTU) and L ‐mimosine on the in vitro growth of Tuber borchii (a white truffle) mycelium. A significant inhibitory effect on mycelium growth was observed for DETC, PTU and L ‐tropolone (0% growth compared to control at 100 μg ml −1 DETC, PTU and L ‐tropolone and at 10 μg ml −1 DETC and L ‐tropolone). As a comparison the action of the same inhibitors was also tested on the growth and pigmentation of the mould Cladosporium sphaerospermum . In the presence of CuSO 4 10 −6 M T. borchii mycelium acquired pigmentation (as rounded aggregates compared to control revealed by SEM microscopy). Tyrosinase activity in the extract from T. borchii mycelium (18‐day culture) was detected spectrophotometrically.