Open AccessAn efficient approach for screening minimal PKS genes from Streptomyces
Author(s) -
MetsäKetelä Mikko,
Salo Virpi,
Halo Laura,
Hautala Anne,
Hakala Juha,
Mäntsälä Pekka,
Ylihonko Kristiina
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08770.x
Subject(s) - polyketide , streptomyces , biology , streptomycetaceae , antibiotics , gene , phylogenetic tree , actinomycetales , dna , genetics , bacteria , biosynthesis
Degenerated oligonucleotide primers were designed to amplify fragments of ketosynthase genes from polyketide antibiotics producing Streptomyces spp. and bacterial strains enriched from soil samples. Cell lysates were used as templates in amplification, so time‐consuming DNA purification was avoided. A phylogenetic tree constructed from the amino acid sequences of the amplified fragments shows a distribution of spore pigments and antibiotics in separate classes. In addition, several different subgroups form within the antibiotics group. Anthracyclines were divided into separate branches according to the starter unit used in biosynthesis.