Open AccessA mutant of Gluconobacter oxydans deficient in gluconic acid dehydrogenase
Author(s) -
Gupta Arun,
Felder Marius,
Verma V.,
Cullum John,
Qazi G.N.
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08769.x
Subject(s) - gluconic acid , biochemistry , mutant , chemistry , dehydrogenase , enzyme , microbiology and biotechnology , biology , gene
Gluconobacter oxydans ATCC 9937 was subjected to transposon mutagenesis using Tn 5 . A non‐pigmented mutant was shown to be defective in gluconic acid dehydrogenase and to produce gluconic acid from glucose, whereas the parent strain produced 2,5‐diketogluconic acid. Cloning and sequencing of the region containing the Tn 5 insertion showed that the insertion point occurred in an open reading frame homologous (42% amino acid identity) to the ribF genes of Pseudomonas fluorescens and Escherichia coli . The resulting lack of a riboflavin cofactor would explain the loss of enzyme activity.