Open AccessPromoter activity of the pER341‐borne ST Phsp in heterologous gene expression in Escherichia coli and Streptococcus thermophilus 1
Author(s) -
Somkuti George A.,
Steinberg Dennis H.
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08759.x
Subject(s) - escherichia coli , streptococcus thermophilus , heterologous , heterologous expression , microbiology and biotechnology , biology , gene , gene expression , genetics , bacteria , recombinant dna , lactobacillus
A 138‐bp Eco O109/ Hin fI fragment of Streptococcus thermophilus plasmid pER341 (2798 bp) including the promoter sequence of the heat stress protein gene hsp16.4 was tested in vector constructs for ability to activate the promoterless green fluorescent protein gene ( gfp ) from a jelly fish in Escherichia coli , S. thermophilus , and lactococci. ST Phsp promoted gfp expression in transformed hosts as evidenced by the presence of green fluorescent (GFP + ) colonies under UV illumination. The results confirmed the potential of ST Phsp as a functional promoter in heterologous gene expression in dairy fermentation bacteria.