Open AccessEffect of specific production rate of recombinant protein on multimerization of plasmid vector and gene expression level
Author(s) -
Saraswat Vibhor,
Kim Dae Young,
Lee Jeewon,
Park YoungHoon
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08751.x
Subject(s) - recombinant dna , escherichia coli , plasmid , growth rate , biology , expression vector , microbiology and biotechnology , fed batch culture , vector (molecular biology) , cell culture , chemistry , gene , biochemistry , fermentation , genetics , geometry , mathematics
In fed‐batch cultures of recombinant Escherichia coli BL21(DE3)[pT7‐G3IL2] at high cell concentration, the post‐induction specific growth rate was carefully regulated by controlled medium feed to maximize the synthesis level of recombinant fusion interleukin‐2, G3·IL‐2. A maximum concentration of G3·IL‐2 (11.25 g l −1 ) was achieved in the induced recombinant culture growing at the rate of 0.056 h −1 . A steep decrease in the expression level of G3·IL‐2 was observed at the post‐induction specific growth rates higher than its optimal value (0.056 h −1 ). In the induced recombinant cultures, plasmid multimerization was observed and highly dependent on specific growth and production rate: a higher post‐induction specific growth rate and an increased specific production rate tended to significantly promote it much further. Moreover, plasmid stability was found to decrease rapidly in a faster growing culture.