Open AccessControl of Azospirillum brasilense NifA activity by P II : effect of replacing Tyr residues of the NifA N‐terminal domain on NifA activity
Author(s) -
Arsène Florence,
Kaminski P.Alexandre,
Elmerich Claudine
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08747.x
Subject(s) - regulator , residue (chemistry) , chemistry , nitrogen fixation , terminal (telecommunication) , azospirillum brasilense , domain (mathematical analysis) , biochemistry , nitrogen , gene , telecommunications , mathematical analysis , mathematics , organic chemistry , computer science
It was previously reported that the N‐terminal domain of Azospirillum brasilense NifA was a negative regulator of the NifA activity and that the P II protein prevented this inhibition under nitrogen fixing conditions. Here, we show that a mutation of a single Tyr residue at position 18 of the N‐terminal domain of NifA led to an active NifA protein that did not require P II for activation under nitrogen fixation conditions.