Open AccessThe yeast Saccharomyces cerevisiae does not sequester chloride but can express a functional mammalian chloride channel
Author(s) -
Coury Larry A.,
McGeoch Julie E.M.,
Guidotti Guido,
Brodsky Jeffrey L.
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08745.x
Subject(s) - chloride channel , yeast , chloride , saccharomyces cerevisiae , chemistry , biochemistry , phosphate , channel blocker , biophysics , biology , organic chemistry , calcium
Chloride uptake into yeast was measured as a function of pH. A small amount of uptake was seen at pH values of 3.0 and 4.0; at pH 6.0 chloride uptake was substantially less than the uptake of phosphate and rubidium. Because chloride uptake is inefficient, we expressed the putative mammalian chloride channel, pI Cln , in yeast and observed a chloride‐selective current when total membrane protein was reconstituted into lipid bilayers. The current was inhibited by a specific chloride channel blocker, 5‐nitro‐2‐(3‐phenylpropylamino)‐benzoic acid. These results suggest that yeast may serve as a means to characterize chloride channels from other organisms.