Open AccessType II restriction endonucleases from Helicobacter pylori include an enzyme with a novel recognition sequence
Author(s) -
Ivic Ana,
Jakeman Kenneth J,
Penn Charles W,
Brown Nigel L
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08724.x
Subject(s) - isoschizomer , ecorv , restriction enzyme , recognition sequence , helicobacter pylori , enzyme , biology , endonuclease , microbiology and biotechnology , biochemistry , chemistry , genetics , hindiii , dna , gene , dna methylation , gene expression
Type II restriction endonuclease activities of Helicobacter pylori strain Roberts and of the type strain H. pylori NCTC 11637 were detected and analysed by conventional techniques. The endonucleases were partially purified, their optima for activity and their recognition and cleavage sites were determined. H. pylori (Roberts) contained at least two enzymes: Hpy BI was an isoschizomer of Rsa I (GT/AC) and Hpy BII was of a novel specificity (GTN/NAC). H. pylori NCTC 11637 was found to contain an isoschizomer of Eco RV ( Hpy CI: GAT/ATC) and at least one other enzyme which was too unstable to characterise.