Open AccessControlled specific expression and purification of 6×His‐tagged proteins in Pseudomonas
Author(s) -
Bertani Iris,
Devescovi Giulia,
Venturi Vittorio
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08714.x
Subject(s) - pseudomonas putida , escherichia coli , pseudomonas , plasmid , biology , bacteria , gene , siderophore , microbiology and biotechnology , biochemistry , genetics
The 6×His affinity tags have proved invaluable for the exclusive purification of proteins, in Escherichia coli , of genes cloned in frame with a 6×His tag and a strong inducible promoter. Here, we demonstrate that the system can be extended to Gram‐negative bacteria other than E. coli , by the use of compatible broad‐host‐range plasmids. As an example, the inducible synthesis and specific purification of the Pseudomonas 6×His‐PfrA siderophore regulatory protein in Pseudomonas putida WCS358 is demonstrated.